PrimePCR™ Probe Assay: MEF2BNB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: MEF2BNB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MEF2BNB, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041711
Assay Design:   exonic
Chromosome Location:   19:19293382-19293482question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000409318 ENST00000376840 ENST00000444486 ENST00000162023 ENST00000602643 ENST00000414043 ENST00000361571 ENST00000572543 ENST00000348066 ENST00000462790 ENST00000588208 ENST00000477565 ENST00000008527 ENST00000286186 ENST00000272879 ENST00000374650 ENST00000346817 ENST00000313728 ENST00000448480 ENST00000360132 ENST00000331817 ENST00000375441 ENST00000304283 ENST00000585773 ENST00000592698 ENST00000296847 ENST00000454449 ENST00000381389 ENST00000434045

Gene Information

Description Not Available

Gene Symbol:   MEF2BNB
Gene Name:   MEF2B neighbor
Aliases:   AK128256, FLJ32599, FLJ46391, MGC189732, MGC189763
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000254901
Entrez:   729991
Chromosome Mapping:   19p13.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.160000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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