PrimePCR™ Probe Assay: CCL3L3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CCL3L3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CCL3L3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0041863
Assay Design:   exonic
Chromosome Location:   17:34523300-34524156question
Amplicon Length:   141
Splice Variants Targeted:   ENST00000308423 ENST00000591562 ENST00000380325 ENST00000378354 ENST00000394484 ENST00000422211 ENST00000542124 ENST00000475153 ENST00000420008 ENST00000259351 ENST00000438723 ENST00000342679 ENST00000361818 ENST00000302262 ENST00000390015 ENST00000565629

Gene Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. This protein binds to several chemokine receptors including chemokine binding protein 2 and chemokine (C-C motif) receptor 5 (CCR5). CCR5 is a co-receptor for HIV and binding of this protein to CCR5 inhibits HIV entry. The copy number of this gene varies among individuals; most individuals have 1-6 copies in the diploid genome although rare individuals have zero or more than six copies. The human genome reference assembly contains two full copies of the gene (CCL3L3 and CCL3L1) and a partial pseudogene. This record represents the more centromeric full-length gene. [provided by RefSeq Apr 2010]

Gene Symbol:   CCL3L3
Gene Name:   chemokine (C-C motif) ligand 3-like 3
Aliases:   464.2, CCL3L1, D17S1718, LD78, LD78BETA, MGC12815, SCYA3L, SCYA3L1
RefSeq:   NC_000017.10 NG_004113.1 NG_023325.1 NT_010799.15
Ensembl:   ENSG00000256515
Entrez:   414062
Chromosome Mapping:   17q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.020000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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