PrimePCR™ Probe Assay: FKBP4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: FKBP4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FKBP4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049145
Assay Design:   exonic
Chromosome Location:   12:2910350-2910441question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000404568 ENST00000393951 ENST00000539181 ENST00000001008 ENST00000383812 ENST00000295981 ENST00000436503 ENST00000403601 ENST00000455057 ENST00000416074 ENST00000277551 ENST00000371372 ENST00000371363 ENST00000371357 ENST00000371355 ENST00000277549 ENST00000397536 ENST00000263257 ENST00000462833 ENST00000491190 ENST00000309879 ENST00000466617 ENST00000272847 ENST00000401399 ENST00000367172 ENST00000367171 ENST00000367170 ENST00000338515 ENST00000339876 ENST00000338586 ENST00000539706 ENST

Gene Information

The protein encoded by this gene is a member of the immunophilin protein family which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A) but unlike FKBP1A this protein does not have immunosuppressant activity when complexed with FK506. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. This encoded protein is known to associate with phytanoyl-CoA alpha-hydroxylase. It can also associate with two heat shock proteins (hsp90 and hsp70) and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors (AAV) resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this encoded protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy. The human genome contains several non-transcribed pseudogenes similar to this gene. [provided by RefSeq Sep 2008]

Gene Symbol:   FKBP4
Gene Name:   FK506 binding protein 4, 59kDa
Aliases:   FKBP51, FKBP52, FKBP59, HBI, Hsp56, PPIase, p52
RefSeq:   NC_000012.11 NT_009759.16
Ensembl:   ENSG00000004478
Entrez:   2288
Chromosome Mapping:   12p13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.920000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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