PrimePCR™ Probe Assay: ST7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $278.00
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PrimePCR™ PreAmp for Probe Assay: ST7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ST7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049153
Assay Design:   exonic
Chromosome Location:   7:116593736-116739896question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000393446 ENST00000265437 ENST00000393451 ENST00000323984 ENST00000393449 ENST00000417919 ENST00000446490 ENST00000421345 ENST00000432298 ENST00000422922 ENST00000449366 ENST00000434836 ENST00000393443 ENST00000465133 ENST00000477742 ENST00000393447 ENST00000393444 ENST00000420755 ENST00000490039

Gene Information

The gene for this product maps to a region on chromosome 7 identified as an autism-susceptibility locus. Mutation screening of the entire coding region in autistic individuals failed to identify phenotype-specific variants suggesting that coding mutations for this gene are unlikely to be involved in the etiology of autism. The function of this gene product has not been determined. Transcript variants encoding different isoforms of this protein have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   ST7
Gene Name:   suppression of tumorigenicity 7
Aliases:   DKFZp762O2113, ETS7q, FAM4A, FAM4A1, HELG, RAY1, SEN4, TSG7
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000004866
Entrez:   7982
Chromosome Mapping:   7q31.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.130000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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