PrimePCR™ Probe Assay: ACSM3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049162
Assay Design:   exonic
Chromosome Location:   16:20781327-20781416question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000568235 ENST00000561584 ENST00000564701 ENST00000289416 ENST00000440284 ENST00000561795

Gene Information

Description Not Available

Gene Symbol:   ACSM3
Gene Name:   acyl-CoA synthetase medium-chain family member 3
Aliases:   SA, SAH
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000005187
Entrez:   6296
Chromosome Mapping:   16p13.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.860000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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