PrimePCR™ Probe Assay: ADIPOR2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049195
Assay Design:   exonic
Chromosome Location:   12:1896958-1897092question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000357103

Gene Information

The adiponectin receptors ADIPOR1 (MIM 607945) and ADIPOR2 serve as receptors for globular and full-length adiponectin (MIM 605441) and mediate increased AMPK (see MIM 602739) and PPAR-alpha (PPARA; MIM 170998) ligand activities as well as fatty acid oxidation and glucose uptake by adiponectin (Yamauchi et al. 2003 [PubMed 12802337]).[supplied by OMIM Mar 2008]

Gene Symbol:   ADIPOR2
Gene Name:   adiponectin receptor 2
Aliases:   ACDCR2, FLJ21432, MGC4640, PAQR2
RefSeq:   NC_000012.11 NT_009759.16
Ensembl:   ENSG00000006831
Entrez:   79602
Chromosome Mapping:   12p13.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.930000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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