PrimePCR™ Probe Assay: MLXIPL, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MLXIPL, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MLXIPL, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049217
Assay Design:   exonic
Chromosome Location:   7:73021992-73030483question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000319080 ENST00000328449 ENST00000414749 ENST00000429400 ENST00000313375 ENST00000354613 ENST00000434326 ENST00000453275 ENST00000456640 ENST00000395189 ENST00000574203 ENST00000573014 ENST00000574926 ENST00000570829 ENST00000575492 ENST00000572858 ENST00000575853 ENST00000570536

Gene Information

This gene encodes a basic helix-loop-helix leucine zipper transcription factor of the Myc/Max/Mad superfamily. This protein forms a heterodimeric complex and binds and activates in a glucose-dependent manner carbohydrate response element (ChoRE) motifs in the promoters of triglyceride synthesis genes. The gene is deleted in Williams-Beuren syndrome a multisystem developmental disorder caused by the deletion of contiguous genes at chromosome 7q11.23. [provided by RefSeq Jul 2008]

Gene Symbol:   MLXIPL
Gene Name:   MLX interacting protein-like
Aliases:   CHREBP, MIO, MONDOB, WBSCR14, WS-bHLH, bHLHd14
RefSeq:   NC_000007.13 NG_009307.1 NT_007933.15
Ensembl:   ENSG00000009950
Entrez:   51085
Chromosome Mapping:   7q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.850000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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