PrimePCR™ Probe Assay: NR1H4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: NR1H4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NR1H4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049245
Assay Design:   exonic
Chromosome Location:   12:100957065-100957210question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000548884 ENST00000392986 ENST00000549996 ENST00000551379 ENST00000188403

Gene Information

This gene encodes a ligand-activated transcription factor which shares structural features in common with nuclear hormone receptor family such as a DNA-binding domain that targets the receptor to specific DNA sequences and a ligand-binding domain which interacts directly with the ligand and contains a ligand-dependent transcriptional activation domain. This protein functions as a receptor for bile acids and when bound to bile acids regulates the expression of genes involved in bile acid synthesis and transport. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Aug 2011]

Gene Symbol:   NR1H4
Gene Name:   nuclear receptor subfamily 1, group H, member 4
Aliases:   BAR, FXR, HRR-1, HRR1, MGC163445, RIP14
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000012504
Entrez:   9971
Chromosome Mapping:   12q23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.640000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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