PrimePCR™ Probe Assay: VEZT, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: VEZT, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: VEZT, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049304
Assay Design:   exonic
Chromosome Location:   12:95650997-95656807question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000407242 ENST00000404995 ENST00000260061 ENST00000596730 ENST00000436874 ENST00000549002 ENST00000551472 ENST00000552821 ENST00000551311 ENST00000546445 ENST00000397792 ENST00000261219 ENST00000407472 ENST00000328554 ENST00000405640 ENST00000512439 ENST00000409581 ENST00000337386 ENST00000409641 ENST00000434473 ENST00000357215 ENST00000545490

Gene Information

This gene encodes a transmembrane protein which has been localized to adherens junctions and shown to bind to myosin VIIA. Examination of expression of this gene in gastric cancer tissues have shown that expression is decreased which appears to be related to hypermethylation of the promoter. Expression of this gene may also be inhibited by binding of a specific microRNA to a target sequence in the 3' UTR of the transcripts. A pseudogene of this gene is located on the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq May 2011]

Gene Symbol:   VEZT
Gene Name:   vezatin, adherens junctions transmembrane protein
Aliases:   DKFZp761C241, VEZATIN
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000028203
Entrez:   55591
Chromosome Mapping:   12q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.970000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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