PrimePCR™ Probe Assay: SYNE2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SYNE2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SYNE2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049414
Assay Design:   exonic
Chromosome Location:   14:64689929-64690066question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000344113 ENST00000554584 ENST00000555002 ENST00000394768 ENST00000555022 ENST00000554805 ENST00000458046 ENST00000441438 ENST00000358025 ENST00000357395

Gene Information

The protein encoded by this gene is a nuclear outer membrane protein that binds cytoplasmic F-actin. This binding tethers the nucleus to the cytoskeleton and aids in the maintenance of the structural integrity of the nucleus. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Mar 2009]

Gene Symbol:   SYNE2
Gene Name:   spectrin repeat containing, nuclear envelope 2
Aliases:   DKFZp434H2235, DKFZp686E01115, DKFZp686H1931, EDMD5, FLJ11014, FLJ43727, FLJ45710, FLJ46790, KIAA1011, NUA, NUANCE, Nesprin-2, SYNE-2, TROPH
RefSeq:   NC_000014.8 NT_026437.12 NG_011535.1 NG_011756.1
Ensembl:   ENSG00000054654
Entrez:   23224
Chromosome Mapping:   14q23.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.630000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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