PrimePCR™ Probe Assay: SP100, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049511
Assay Design:   exonic
Chromosome Location:   2:231282367-231307803question
Amplicon Length:   136
Splice Variants Targeted:   ENST00000301146 ENST00000291764 ENST00000330436 ENST00000400454 ENST00000404019 ENST00000404537 ENST00000373876 ENST00000265318 ENST00000486687 ENST00000444636 ENST00000415673 ENST00000458341 ENST00000243810 ENST00000396563 ENST00000264052 ENST00000432979 ENST00000409824 ENST00000409341 ENST00000409112 ENST00000340126 ENST00000409897 ENST00000427101 ENST00000341950

Gene Information

This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis immunity and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein. [provided by RefSeq Aug 2011]

Gene Symbol:   SP100
Gene Name:   SP100 nuclear antigen
Aliases:   DKFZp686E07254, FLJ00340, FLJ34579
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000067066
Entrez:   6672
Chromosome Mapping:   2q37.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.430000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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