PrimePCR™ Probe Assay: SYT1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SYT1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SYT1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049525
Assay Design:   exonic
Chromosome Location:   12:79611342-79611449question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000446242 ENST00000552624 ENST00000552744 ENST00000551304 ENST00000549671 ENST00000547046 ENST00000552074 ENST00000457153 ENST00000261205 ENST00000393240 ENST00000301295 ENST00000587891 ENST00000589437 ENST00000346986 ENST00000367400 ENST00000367399 ENST00000538660 ENST00000535699 ENST00000544212

Gene Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al. 2001 [PubMed 11242035]).[supplied by OMIM Jul 2010]

Gene Symbol:   SYT1
Gene Name:   synaptotagmin I
Aliases:   DKFZp781D2042, P65, SVP65, SYT
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000067715
Entrez:   6857
Chromosome Mapping:   12cen-q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.210000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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