PrimePCR™ Probe Assay: GAL, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049543
Assay Design:   exonic
Chromosome Location:   11:68458492-68458589question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000265643

Gene Information

Galanin is small neuropeptide that functions as a cellular messenger within the central and peripheral nervous systems modulating diverse physiologic functions (Mechenthaler 2008 [PubMed 18500643]).[supplied by OMIM Jan 2009]

Gene Symbol:   GAL
Gene Name:   galanin prepropeptide
Aliases:   GALN, GLNN, GMAP, MGC40167
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000069482
Entrez:   51083
Chromosome Mapping:   11q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.550000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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