PrimePCR™ Probe Assay: PTPN21, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PTPN21, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PTPN21, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049561
Assay Design:   exonic
Chromosome Location:   14:88934251-88934394question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000556564 ENST00000553531 ENST00000328736

Gene Information

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth differentiation mitotic cycle and oncogenic transformation. This PTP contains an N-terminal domain similar to cytoskeletal- associated proteins including band 4.1 ezrin merlin and radixin. This PTP was shown to specially interact with BMX/ETK a member of Tec tyrosine kinase family characterized by a multimodular structures including PH SH3 and SH2 domains. The interaction of this PTP with BMX kinase was found to increase the activation of STAT3 but not STAT2 kinase. Studies of the similar gene in mice suggested the possible roles of this PTP in liver regeneration and spermatogenesis. [provided by RefSeq Jul 2008]

Gene Symbol:   PTPN21
Gene Name:   protein tyrosine phosphatase, non-receptor type 21
Aliases:   PTPD1, PTPRL10
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000070778
Entrez:   11099
Chromosome Mapping:   14q31.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.000000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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