PrimePCR™ Probe Assay: ING3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ING3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ING3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049569
Assay Design:   exonic
Chromosome Location:   7:120590881-120591235question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000315870 ENST00000339121 ENST00000445699

Gene Information

The protein encoded by this gene is similar to ING1 a tumor suppressor protein that can interact with TP53 inhibit cell growth and induce apoptosis. This protein contains a PHD-finger which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters including promoters of p21/waf1 and bax. Overexpression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Two alternatively spliced transcript variants encoding different isoforms have been observed. [provided by RefSeq Jul 2008]

Gene Symbol:   ING3
Gene Name:   inhibitor of growth family, member 3
Aliases:   Eaf4, FLJ20089, ING2, MEAF4, p47ING3
RefSeq:   NC_000007.13 NG_023322.1 NT_007933.15
Ensembl:   ENSG00000071243
Entrez:   54556
Chromosome Mapping:   7q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.830000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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