PrimePCR™ Probe Assay: SMARCE1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SMARCE1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SMARCE1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049598
Assay Design:   exonic
Chromosome Location:   17:38792670-38792776question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000311181 ENST00000590714 ENST00000348513 ENST00000431889 ENST00000377808 ENST00000578044 ENST00000580419 ENST00000400122 ENST00000478349 ENST00000580654 ENST00000577721 ENST00000582955 ENST00000544009

Gene Information

The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein either alone or when in the SWI/SNF complex can bind to 4-way junction DNA which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins this protein has no yeast counterpart. [provided by RefSeq Jul 2008]

Gene Symbol:   SMARCE1
Gene Name:   SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
Aliases:   BAF57, FLJ35648
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000073584
Entrez:   6605
Chromosome Mapping:   17q21.2

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