PrimePCR™ Probe Assay: DYNC1I2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $220.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049661
Assay Design:   exonic
Chromosome Location:   2:172549402-172563877question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000452242 ENST00000340296 ENST00000397119 ENST00000410079 ENST00000438879 ENST00000508530 ENST00000409197 ENST00000456808 ENST00000409317 ENST00000409773 ENST00000411953 ENST00000409453 ENST00000435234 ENST00000443458 ENST00000412370 ENST00000423910 ENST00000425485 ENST00000430778 ENST00000422646 ENST00000534253 ENST00000263811 ENST00000358002 ENST00000268712 ENST00000395849 ENST00000395857

Gene Information

Description Not Available

Gene Symbol:   DYNC1I2
Gene Name:   dynein, cytoplasmic 1, intermediate chain 2
Aliases:   DNCI2, FLJ21089, FLJ90842, IC2, MGC104199, MGC111094, MGC9324
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000077380
Entrez:   1781
Chromosome Mapping:   2q31.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.380000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download