PrimePCR™ Probe Assay: MID2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MID2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MID2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049716
Assay Design:   exonic
Chromosome Location:   X:107084439-107084561question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000451923 ENST00000262843 ENST00000443968

Gene Information

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains a RING a B-box type 1 and a B-box type 2 and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq Feb 2009]

Gene Symbol:   MID2
Gene Name:   midline 2
Aliases:   FLJ37715, FLJ41813, FXY2, RNF60, TRIM1
RefSeq:   NC_000023.10 NG_011907.1 NT_011651.17
Ensembl:   ENSG00000080561
Entrez:   11043
Chromosome Mapping:   Xq22.3

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