PrimePCR™ Probe Assay: PLOD1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PLOD1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PLOD1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049759
Assay Design:   exonic
Chromosome Location:   1:12010477-12010569question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000344275 ENST00000442833 ENST00000280560 ENST00000392439 ENST00000542678 ENST00000346530 ENST00000442028 ENST00000381971 ENST00000339468 ENST00000449038 ENST00000429000 ENST00000196061 ENST00000376369 ENST00000312358 ENST00000396698 ENST00000396695

Gene Information

Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. [provided by RefSeq Jul 2008]

Gene Symbol:   PLOD1
Gene Name:   procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1
Aliases:   FLJ42041, LH, LH1, LLH, PLOD
RefSeq:   NC_000001.10 NG_007945.1 NG_008159.1 NT_021937.19
Ensembl:   ENSG00000083444
Entrez:   5351
Chromosome Mapping:   1p36.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 37.720000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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