PrimePCR™ Probe Assay: THPO, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: THPO, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: THPO, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049874
Assay Design:   exonic
Chromosome Location:   3:184090191-184090340question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000204615 ENST00000445696 ENST00000421442

Gene Information

Megakaryocytopoiesis is the cellular development process that leads to platelet production. The protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL the product of myeloproliferative leukemia virus oncogene. Alternate splicing results in multiple transcript variants of this gene.[provided by RefSeq Apr 2010]

Gene Symbol:   THPO
Gene Name:   thrombopoietin
Aliases:   MGC163194, MGDF, MKCSF, ML, MPLLG, TPO
RefSeq:   NC_000003.11 NG_012136.1 NT_005612.16
Ensembl:   ENSG00000090534
Entrez:   7066
Chromosome Mapping:   3q27

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.520000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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