PrimePCR™ Probe Assay: CDHR5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CDHR5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: CDHR5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0049981
Assay Design:   exonic
Chromosome Location:   11:624631-624819question
Amplicon Length:   74
Splice Variants Targeted:   ENST00000372067 ENST00000324450 ENST00000417285 ENST00000413642 ENST00000372055 ENST00000482630 ENST00000425836 ENST00000372064 ENST00000372077 ENST00000519767 ENST00000520948 ENST00000523873 ENST00000523950 ENST00000457104 ENST00000518689 ENST00000523125 ENST00000518824 ENST00000230480 ENST00000520265 ENST00000358353 ENST00000349570 ENST00000526077 ENST00000534311 ENST00000531088 ENST00000397542 ENST00000255858 ENST00000540456 ENST00000392772 ENST00000252744 ENST00000215582 ENST00000370507 ENST

Gene Information

This gene is a novel mucin-like gene that is a member of the cadherin superfamily. While encoding nonpolymorphic tandem repeats rich in proline serine and threonine similar to mucin proteins the gene also contains sequence encoding calcium-binding motifs found in all cadherins. The role of the hybrid extracellular region and the specific function of this protein have not yet been determined. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq Jan 2010]

Gene Symbol:   CDHR5
Gene Name:   cadherin-related family member 5
Aliases:   FLJ20219, MU-PCDH, MUCDHL, MUPCDH
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000099834
Entrez:   53841
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.670000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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