PrimePCR™ Probe Assay: DHRS7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: DHRS7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DHRS7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050073
Assay Design:   exonic
Chromosome Location:   14:60616159-60616795question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000216500 ENST00000557751 ENST00000536410 ENST00000554101 ENST00000557185

Gene Information

Short-chain dehydrogenases/reductases (SDRs) such as DHRS7 catalyze the oxidation/reduction of a wide range of substrates including retinoids and steroids (Haeseleer and Palczewski 2000 [PubMed 10800688]).[supplied by OMIM Jun 2009]

Gene Symbol:   DHRS7
Gene Name:   dehydrogenase/reductase (SDR family) member 7
Aliases:   SDR34C1, retDSR4, retSDR4
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000100612
Entrez:   51635
Chromosome Mapping:   14q23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.270000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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