PrimePCR™ Probe Assay: PFDN4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PFDN4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PFDN4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050103
Assay Design:   exonic
Chromosome Location:   20:52836186-52836317question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000371419

Gene Information

This gene encodes a member of the prefoldin beta subunit family. The encoded protein is one of six subunits of prefoldin a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides thereby allowing them to fold correctly. The complex consisting of two alpha and four beta subunits forms a double beta barrel assembly with six protruding coiled-coils. [provided by RefSeq Jul 2008]

Gene Symbol:   PFDN4
Gene Name:   prefoldin subunit 4
Aliases:   C1, PFD4
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000101132
Entrez:   5203
Chromosome Mapping:   20q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.530000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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