PrimePCR™ Probe Assay: EEF1A2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: EEF1A2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EEF1A2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050109
Assay Design:   exonic
Chromosome Location:   20:62126441-62127304question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000279027 ENST00000472148 ENST00000290317 ENST00000396360 ENST00000435032 ENST00000298049 ENST00000217182

Gene Information

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain heart and skeletal muscle and the other isoform (alpha 1) is expressed in brain placenta lung liver kidney and pancreas. This gene may be critical in the development of ovarian cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2008]

Gene Symbol:   EEF1A2
Gene Name:   eukaryotic translation elongation factor 1 alpha 2
Aliases:   EEF1AL, EF-1-alpha-2, EF1A, FLJ41696, HS1, STN, STNL
RefSeq:   NC_000020.10 NT_011333.6
Ensembl:   ENSG00000101210
Entrez:   1917
Chromosome Mapping:   20q13.3

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