PrimePCR™ Probe Assay: SYP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SYP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SYP, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050159
Assay Design:   exonic
Chromosome Location:   X:49047960-49048180question
Amplicon Length:   191
Splice Variants Targeted:   ENST00000263233 ENST00000472598 ENST00000479808 ENST00000538567 ENST00000409874 ENST00000339063 ENST00000397679 ENST00000359428 ENST00000411637 ENST00000483497 ENST00000451030 ENST00000372189 ENST00000372195 ENST00000372190 ENST00000599440 ENST00000596664 ENST00000601081 ENST00000594524

Gene Information

This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR). [provided by RefSeq Aug 2011]

Gene Symbol:   SYP
Gene Name:   synaptophysin
Aliases:   MRXSYP
RefSeq:   NC_000023.10 NG_012532.1 NG_017135.1 NT_079573.4
Ensembl:   ENSG00000102003
Entrez:   6855
Chromosome Mapping:   Xp11.23-p11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996500
y-intercept 36.720000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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