PrimePCR™ Probe Assay: PLS3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PLS3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PLS3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050161
Assay Design:   exonic
Chromosome Location:   X:114877691-114879356question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000355899 ENST00000289290 ENST00000497870 ENST00000420625 ENST00000537301 ENST00000539310 ENST00000602045 ENST00000599970 ENST00000593390 ENST00000595829 ENST00000596511 ENST00000594997

Gene Information

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts endothelial cells epithelial cells melanocytes etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants.[provided by RefSeq Feb 2010]

Gene Symbol:   PLS3
Gene Name:   plastin 3
Aliases:   T-plastin
RefSeq:   NC_000023.10 NT_028405.12 NG_012518.1
Ensembl:   ENSG00000102024
Entrez:   5358
Chromosome Mapping:   Xq23

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