PrimePCR™ Probe Assay: SIGLEC5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SIGLEC5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SIGLEC5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050337
Assay Design:   exonic
Chromosome Location:   19:52115207-52115314question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000534261 ENST00000222107 ENST00000570106

Gene Information

This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion. [provided by RefSeq Feb 2012]

Gene Symbol:   SIGLEC5
Gene Name:   sialic acid binding Ig-like lectin 5
Aliases:   CD170, CD33L2, OB-BP2, OBBP2, SIGLEC-5
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000105501
Entrez:   8778
Chromosome Mapping:   19q13.3

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