PrimePCR™ Probe Assay: ATP4A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ATP4A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ATP4A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050348
Assay Design:   exonic
Chromosome Location:   19:36045875-36045976question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000262623

Gene Information

The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+ K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes a catalytic alpha subunit of the gastric H+ K+-ATPase. [provided by RefSeq Jul 2008]

Gene Symbol:   ATP4A
Gene Name:   ATPase, H+/K+ exchanging, alpha polypeptide
Aliases:   ATP6A
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000105675
Entrez:   495
Chromosome Mapping:   19q13.1

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