PrimePCR™ Probe Assay: PON2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PON2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PON2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050367
Assay Design:   exonic
Chromosome Location:   7:95034690-95034786question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000536183 ENST00000222572 ENST00000433091

Gene Information

This gene encodes a member of the paraoxonase gene family which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues membrane-bound and may act as a cellular antioxidant protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones important bacterial quorum-sensing mediators suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   PON2
Gene Name:   paraoxonase 2
Aliases:   Not Available
RefSeq:   NC_000007.13 NG_008725.1 NT_007933.15
Ensembl:   ENSG00000105854
Entrez:   5445
Chromosome Mapping:   7q21.3

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