PrimePCR™ Probe Assay: MEST, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MEST, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MEST, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050414
Assay Design:   exonic
Chromosome Location:   7:130135209-130135342question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000409479 ENST00000585421 ENST00000536189 ENST00000262768 ENST00000586057 ENST00000250916 ENST00000336798 ENST00000339394 ENST00000338962 ENST00000289805 ENST00000335360 ENST00000298229 ENST00000541756 ENST00000524189 ENST00000341441 ENST00000427521 ENST00000416162 ENST00000378576 ENST00000399874 ENST00000433159 ENST00000421001 ENST00000223215 ENST00000437945 ENST00000437637 ENST00000458161 ENST00000393187 ENST00000414468 ENST00000413692 ENST00000424781 ENST00000539237 ENST00000250373 ENST

Gene Information

This gene encodes a member of the alpha/beta hydrolase superfamily. It is imprinted exhibiting preferential expression from the paternal allele in fetal tissues and isoform-specific imprinting in lymphocytes. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3 and 4 and the long arm of chromosomes 6 and 15. [provided by RefSeq Dec 2011]

Gene Symbol:   MEST
Gene Name:   mesoderm specific transcript homolog (mouse)
Aliases:   DKFZp686L18234, MGC111102, MGC8703, PEG1
RefSeq:   NC_000007.13 NT_007933.15 NG_009226.1
Ensembl:   ENSG00000106484
Entrez:   4232
Chromosome Mapping:   7q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.970000
Efficiency 106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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