PrimePCR™ Probe Assay: SLC1A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SLC1A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SLC1A1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050428
Assay Design:   exonic
Chromosome Location:   9:4585435-4585550question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000262352 ENST00000422398

Gene Information

This gene encodes a member of the high-affinity glutamate transporters that play an essential role in transporting glutamate across plasma membranes. In brain these transporters are crucial in terminating the postsynaptic action of the neurotransmitter glutamate and in maintaining extracellular glutamate concentrations below neurotoxic levels. This transporter also transports aspartate and mutations in this gene are thought to cause dicarboxylicamino aciduria also known as glutamate-aspartate transport defect. [provided by RefSeq Mar 2010]

Gene Symbol:   SLC1A1
Gene Name:   solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1
Aliases:   EAAC1, EAAT3
RefSeq:   NC_000009.11 NG_017044.1 NT_008413.18
Ensembl:   ENSG00000106688
Entrez:   6505
Chromosome Mapping:   9p24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.440000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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