PrimePCR™ Probe Assay: SGCA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SGCA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SGCA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050511
Assay Design:   exonic
Chromosome Location:   17:48252623-48252744question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000344627 ENST00000262018 ENST00000511303 ENST00000504073 ENST00000543315

Gene Information

This gene encodes a component of the dystrophin-glycoprotein complex (DGC) which is critical to the stability of muscle fiber membranes and to the linking of the actin cytoskeleton to the extracellular matrix. Its expression is thought to be restricted to striated muscle. Mutations in this gene result in type 2D autosomal recessive limb-girdle muscular dystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2008]

Gene Symbol:   SGCA
Gene Name:   sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein)
Aliases:   50-DAG, A2, ADL, DAG2, DMDA2, LGMD2D, SCARMD1, adhalin
RefSeq:   NC_000017.10 NG_008889.1 NT_010783.15
Ensembl:   ENSG00000108823
Entrez:   6442
Chromosome Mapping:   17q21

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