PrimePCR™ Probe Assay: SLC11A2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SLC11A2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SLC11A2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050600
Assay Design:   exonic
Chromosome Location:   12:51398580-51399105question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000309863 ENST00000262051 ENST00000547198 ENST00000262052 ENST00000547688 ENST00000541174 ENST00000545993 ENST00000546743 ENST00000547579 ENST00000550714 ENST00000547732 ENST00000549110 ENST00000548193 ENST00000394904 ENST00000367565 ENST00000369443 ENST00000369442 ENST00000299969 ENST00000319327 ENST00000539332

Gene Information

This gene encodes a member of the solute carrier family 11 protein family. The product of this gene transports divalent metals and is involved in iron absorption. Mutations in this gene are associated with hypochromic microcytic anemia with iron overload. A related solute carrier family 11 protein gene is located on chromosome 2. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Apr 2010]

Gene Symbol:   SLC11A2
Gene Name:   solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2
Aliases:   DCT1, DMT1, FLJ37416, NRAMP2
RefSeq:   NC_000012.11 NT_029419.12 NG_021139.1
Ensembl:   ENSG00000110911
Entrez:   4891
Chromosome Mapping:   12q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.770000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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