PrimePCR™ Probe Assay: SLC6A12, Human

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.



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PrimePCR™ PreAmp for Probe Assay: SLC6A12, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for Probe Assay: SLC6A12, Human
Reaction: 200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.

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Assay Information

Technology:		qPCR
Assay Type:		Probe
Application:		Gene Expression
Unique Assay ID:		qHsaCEP0050612
Assay Design:		exonic
Chromosome Location:		12:312045-313827question
Amplicon Length:		70
Splice Variants Targeted:		ENST00000359674 ENST00000424061 ENST00000536824 ENST00000537793 ENST00000535347 ENST00000397296 ENST00000428720 ENST00000305892 ENST00000544117 ENST00000547026 ENST00000548932 ENST00000301042 ENST00000483722 ENST00000296456 ENST00000438011 ENST00000322107 ENST00000399397 ENST00000342528 ENST00000399391 ENST00000252032 ENST00000323327 ENST00000481110 ENST00000260795 ENST00000352904 ENST00000340107 ENST00000440486 ENST00000412135 ENST00000361924 ENST00000356847 ENST00000450863 ENST00000591680 ENST

Gene Information

Description Not Available

Gene Symbol:		SLC6A12
Gene Name:		solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12
Aliases:		BGT-1, BGT1, FLJ38727, GAT2
RefSeq:		NC_000012.11 NT_009759.16
Ensembl:		ENSG00000111181
Entrez:		6539
Chromosome Mapping:		12p13

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999900
y-intercept	35.570000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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