PrimePCR™ Probe Assay: MSH3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MSH3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MSH3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050750
Assay Design:   exonic
Chromosome Location:   5:80171990-80172113question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000265081

Gene Information

The protein encoded by this gene forms a heterodimer with MSH2 to form MutS beta part of the post-replicative DNA mismatch repair system. MutS beta initiates mismatch repair by binding to a mismatch and then forming a complex with MutL alpha heterodimer. This gene contains a polymorphic 9 bp tandem repeat sequence in the first exon. The repeat is present 6 times in the reference genome sequence and 3-7 repeats have been reported. Defects in this gene are a cause of susceptibility to endometrial cancer. [provided by RefSeq Mar 2011]

Gene Symbol:   MSH3
Gene Name:   mutS homolog 3 (E. coli)
Aliases:   DUP, MGC163306, MGC163308, MRP1
RefSeq:   NG_023304.1 NC_000005.9 NG_016607.1 NT_006713.15
Ensembl:   ENSG00000113318
Entrez:   4437
Chromosome Mapping:   5q11-q12

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