PrimePCR™ Probe Assay: CSNK1A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050776
Assay Design:   exonic
Chromosome Location:   5:148892638-148892746question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000379800 ENST00000261798 ENST00000504676 ENST00000515435 ENST00000515768 ENST00000503350 ENST00000377843

Gene Information

Description Not Available

Gene Symbol:   CSNK1A1
Gene Name:   casein kinase 1, alpha 1
Aliases:   CK1, HLCDGP1, PRO2975
RefSeq:   NC_000005.9 NT_029289.11
Ensembl:   ENSG00000113712
Entrez:   1452
Chromosome Mapping:   5q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.130000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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