PrimePCR™ Probe Assay: ZNF346, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ZNF346, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF346, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050781
Assay Design:   exonic
Chromosome Location:   5:176489062-176491516question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000506693 ENST00000358149 ENST00000512315 ENST00000503425 ENST00000511834 ENST00000503039 ENST00000261948

Gene Information

The protein encoded by this gene is a nucleolar zinc finger protein that preferentially binds to double-stranded (ds) RNA or RNA/DNA hybrids rather than DNA alone. Mutational studies indicate that the zinc finger domains are not only essential for dsRNA binding but are also required for its nucleolar localization. The encoded protein may be involved in cell growth and survival. [provided by RefSeq Jul 2008]

Gene Symbol:   ZNF346
Gene Name:   zinc finger protein 346
Aliases:   DKFZp547M223, JAZ, Zfp346
RefSeq:   NC_000005.9 NT_023133.13
Ensembl:   ENSG00000113761
Entrez:   23567
Chromosome Mapping:   5q35.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.550000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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