PrimePCR™ Probe Assay: PCCB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PCCB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PCCB, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050794
Assay Design:   exonic
Chromosome Location:   3:135974788-135975464question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000370867 ENST00000457880 ENST00000319194 ENST00000319212 ENST00000525109 ENST00000441424 ENST00000251654 ENST00000490504 ENST00000483687 ENST00000468777 ENST00000466072 ENST00000471595 ENST00000469217 ENST00000465423 ENST00000478469 ENST00000462542 ENST00000494742 ENST00000459873 ENST00000306151 ENST00000375254 ENST00000425413 ENST00000417040 ENST00000375267 ENST00000375217 ENST00000375226 ENST00000268138 ENST00000560985 ENST00000449082

Gene Information

The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2). Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq May 2010]

Gene Symbol:   PCCB
Gene Name:   propionyl CoA carboxylase, beta polypeptide
Aliases:   DKFZp451E113
RefSeq:   NC_000003.11 NG_008939.1 NT_005612.16
Ensembl:   ENSG00000114054
Entrez:   5096
Chromosome Mapping:   3q21-q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.760000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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