PrimePCR™ Probe Assay: MRPL19, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MRPL19, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MRPL19, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050867
Assay Design:   exonic
Chromosome Location:   2:75882280-75882414question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000393909 ENST00000409374 ENST00000453233

Gene Information

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species the proteins comprising the mitoribosome differ greatly in sequence and sometimes in biochemical properties which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq Jul 2008]

Gene Symbol:   MRPL19
Gene Name:   mitochondrial ribosomal protein L19
Aliases:   KIAA0104, L19mt, MGC20675, MRP-L15, MRP-L19, MRPL15, RLX1, RPML15
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000115364
Entrez:   9801
Chromosome Mapping:   2q11.1-q11.2

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