PrimePCR™ Probe Assay: ERRFI1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ERRFI1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ERRFI1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0050942
Assay Design:   exonic
Chromosome Location:   1:8073966-8074109question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000377482 ENST00000467067 ENST00000469499

Gene Information

ERRFI1 is a cytoplasmic protein whose expression is upregulated with cell growth (Wick et al. 1995 [PubMed 7641805]). It shares significant homology with the protein product of rat gene-33 which is induced during cell stress and mediates cell signaling (Makkinje et al. 2000 [PubMed 10749885]; Fiorentino et al. 2000 [PubMed 11003669]).[supplied by OMIM Mar 2008]

Gene Symbol:   ERRFI1
Gene Name:   ERBB receptor feedback inhibitor 1
Aliases:   GENE-33, MIG-6, MIG6, RALT
RefSeq:   NC_000001.10 NT_021937.19
Ensembl:   ENSG00000116285
Entrez:   54206
Chromosome Mapping:   1p36

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