PrimePCR™ Probe Assay: ELOVL4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ELOVL4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ELOVL4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051037
Assay Design:   exonic
Chromosome Location:   6:80625323-80625470question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000369816

Gene Information

This gene encodes a membrane-bound protein which is a member of the ELO family proteins which participate in the biosynthesis of fatty acids. Consistent with the expression of the encoded protein in photoreceptor cells of the retina mutations and small deletions in this gene are associated with Stargardt-like macular dystrophy (STGD3) and autosomal dominant Stargardt-like macular dystrophy (ADMD) also referred to as autosomal dominant atrophic macular degeneration. [provided by RefSeq Jul 2008]

Gene Symbol:   ELOVL4
Gene Name:   elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 4
Aliases:   ADMD, CT118, FLJ17667, FLJ92876, STGD2, STGD3
RefSeq:   NC_000006.11 NG_009108.1 NT_007299.13
Ensembl:   ENSG00000118402
Entrez:   6785
Chromosome Mapping:   6q14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.540000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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