PrimePCR™ Probe Assay: SPOP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SPOP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SPOP, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051155
Assay Design:   exonic
Chromosome Location:   17:47699414-47700220question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000393328 ENST00000504102 ENST00000503676 ENST00000509079 ENST00000505581 ENST00000507970 ENST00000514121 ENST00000515508 ENST00000510476 ENST00000508805 ENST00000451526 ENST00000393331 ENST00000347630

Gene Information

This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX) which interacts with histone deacetylase core histones and other histone-associated proteins. In mouse the encoded protein binds to the putative leucine zipper domain of macroH2A1.2 a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq Jul 2008]

Gene Symbol:   SPOP
Gene Name:   speckle-type POZ protein
Aliases:   TEF2
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000121067
Entrez:   8405
Chromosome Mapping:   17q21.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.220000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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