PrimePCR™ Probe Assay: FKBP9, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
Your Price:   Log In
Quantity:  
 

PrimePCR™ PreAmp for Probe Assay: FKBP9, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FKBP9, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051216
Assay Design:   exonic
Chromosome Location:   7:33035814-33035904question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000575445 ENST00000423312 ENST00000315264 ENST00000266031 ENST00000218758 ENST00000592828 ENST00000503128 ENST00000398261 ENST00000228606 ENST00000373095 ENST00000373084 ENST00000242209 ENST00000418354 ENST00000538336 ENST00000538443 ENST00000490776 ENST00000395527 ENST00000261503 ENST00000395530 ENST00000581399 ENST00000536879

Gene Information

Description Not Available

Gene Symbol:   FKBP9
Gene Name:   FK506 binding protein 9, 63 kDa
Aliases:   DKFZp586B1723, FKBP60, FKBP63, MGC126772, MGC138258, PPIase
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000122642
Entrez:   11328
Chromosome Mapping:   7p11.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.010000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download