PrimePCR™ Probe Assay: SPATS2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SPATS2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SPATS2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051245
Assay Design:   exonic
Chromosome Location:   12:49854619-49854746question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000550997 ENST00000549538 ENST00000548654 ENST00000550643 ENST00000548710 ENST00000549179 ENST00000548377 ENST00000549298 ENST00000553127 ENST00000321898 ENST00000551540 ENST00000552918 ENST00000548777 ENST00000547865 ENST00000552171

Gene Information

Description Not Available

Gene Symbol:   SPATS2
Gene Name:   spermatogenesis associated, serine-rich 2
Aliases:   FLJ13117, P59SCR, SCR59, SPATA10
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000123352
Entrez:   65244
Chromosome Mapping:   12q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 37.710000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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