PrimePCR™ Probe Assay: EEF1E1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: EEF1E1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EEF1E1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051328
Assay Design:   exonic
Chromosome Location:   6:8097623-8102688question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000429723 ENST00000502429 ENST00000379715 ENST00000507463 ENST00000488226

Gene Information

This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However its mouse homolog has been shown to translocate to the nucleus in response to DNA damage and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream MUTED (muted homolog) gene. An EEF1E1-related pseudogene has been identified on chromosome 2. [provided by RefSeq Dec 2010]

Gene Symbol:   EEF1E1
Gene Name:   eukaryotic translation elongation factor 1 epsilon 1
Aliases:   AIMP3, P18
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000124802
Entrez:   9521
Chromosome Mapping:   6p24.3

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