PrimePCR™ Probe Assay: OPRL1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: OPRL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: OPRL1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051349
Assay Design:   exonic
Chromosome Location:   20:62729976-62730065question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000336866 ENST00000355631 ENST00000349451

Gene Information

The protein encoded by this gene is a G protein-coupled receptor whose expression can be induced by phytohemagglutinin. The encoded integral membrane protein is a receptor for the 17 aa neuropeptide nociceptin/orphanin FQ. This gene may be involved in the regulation of numerous brain activities particularly instinctive and emotional behaviors. A promoter for this gene also functions as a promoter for another gene regulator of G-protein signalling 19 (RGS19) located on the opposite strand. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jan 2011]

Gene Symbol:   OPRL1
Gene Name:   opiate receptor-like 1
Aliases:   KOR-3, MGC34578, NOCIR, OOR, ORL1
RefSeq:   NC_000020.10 NT_011333.6
Ensembl:   ENSG00000125510
Entrez:   4987
Chromosome Mapping:   20q13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.060000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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