PrimePCR™ Probe Assay: AVPR2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: AVPR2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AVPR2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051426
Assay Design:   exonic
Chromosome Location:   X:153171567-153171679question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000356341 ENST00000530617 ENST00000430276 ENST00000373950 ENST00000389054 ENST00000398557 ENST00000253811 ENST00000529487 ENST00000375202 ENST00000588430 ENST00000264436 ENST00000407644 ENST00000355733 ENST00000413157 ENST00000430656 ENST00000377813 ENST00000360807 ENST00000377807 ENST00000246043 ENST00000376351 ENST00000404767 ENST00000389470 ENST00000358927 ENST00000430697 ENST00000370049 ENST00000337474 ENST00000309424 ENST00000331079 ENST00000437750 ENST00000596997 ENST00000595875 ENST

Gene Information

This gene encodes the vasopressin receptor type 2 also known as the V2 receptor which belongs to the seven-transmembrane-domain G protein-coupled receptor (GPCR) superfamily and couples to Gs thus stimulating adenylate cyclase. The subfamily that includes the V2 receptor the V1a and V1b vasopressin receptors the oxytocin receptor and isotocin and mesotocin receptors in non-mammals is well conserved though several members signal via other G proteins. All bind similar cyclic nonapeptide hormones. The V2 receptor is expressed in the kidney tubule predominantly in the distal convoluted tubule and collecting ducts where its primary property is to respond to the pituitary hormone arginine vasopressin (AVP) by stimulating mechanisms that concentrate the urine and maintain water homeostasis in the organism. When the function of this gene is lost the disease Nephrogenic Diabetes Insipidus (NDI) results. The V2 receptor is also expressed outside the kidney although its tissue localization is uncertain. When these 'extrarenal receptors' are stimulated by infusion of a V2 selective agonist (dDAVP) a variety of clotting factors are released into the bloodstream. The physiologic importance of this property is not known - its absence does not appear to be detrimental in NDI patients. The gene expression has also been described in fetal lung tissue and lung cancer associated with alternative splicing. [provided by RefSeq Jul 2008]

Gene Symbol:   AVPR2
Gene Name:   arginine vasopressin receptor 2
Aliases:   ADHR, DI1, DIR, DIR3, MGC126533, MGC138386, NDI, V2R
RefSeq:   NC_000023.10 NG_008687.1 NG_013220.1 NT_167198.1
Ensembl:   ENSG00000126895
Entrez:   554
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.100000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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