PrimePCR™ Probe Assay: ZNF780B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ZNF780B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF780B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051463
Assay Design:   exonic
Chromosome Location:   19:40553363-40554655question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000570495 ENST00000563169 ENST00000564323 ENST00000562156 ENST00000573685 ENST00000573113 ENST00000398253 ENST00000434248 ENST00000221355 ENST00000598845 ENST00000593605 ENST00000450241 ENST00000414720 ENST00000594395 ENST00000595773 ENST00000599972 ENST00000455521 ENST00000595687 ENST00000340963 ENST00000427179 ENST00000394226 ENST00000446148 ENST00000274599 ENST00000418587 ENST00000590751 ENST00000593133 ENST00000451802 ENST00000358744 ENST00000590390 ENST00000328550 ENST00000376436 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF780B
Gene Name:   zinc finger protein 780B
Aliases:   ZNF779
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000128000
Entrez:   163131
Chromosome Mapping:   19q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.830000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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