PrimePCR™ Probe Assay: PDE11A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PDE11A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PDE11A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051490
Assay Design:   exonic
Chromosome Location:   2:178705013-178740609question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000286063 ENST00000358450 ENST00000409504 ENST00000389683 ENST00000433879 ENST00000449286

Gene Information

The 3'5'-cyclic nucleotides cAMP and cGMP function as second messengers in a wide variety of signal transduction pathways. 3'5'-cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of cAMP and cGMP to the corresponding 5'-monophosphates and provide a mechanism to downregulate cAMP and cGMP signaling. This gene encodes a member of the PDE protein superfamily. Mutations in this gene are a cause of Cushing disease and adrenocortical hyperplasia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   PDE11A
Gene Name:   phosphodiesterase 11A
Aliases:   FLJ23693, MGC133355, MGC133356, PPNAD2
RefSeq:   NC_000002.11 NT_005403.17 NG_012168.1
Ensembl:   ENSG00000128655
Entrez:   50940
Chromosome Mapping:   2q31.2

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