PrimePCR™ Probe Assay: DUT, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: DUT, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DUT, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0051502
Assay Design:   exonic
Chromosome Location:   15:48624602-48626705question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000559935 ENST00000559416 ENST00000558813 ENST00000331200 ENST00000558472 ENST00000455976 ENST00000559540 ENST00000450740 ENST00000558367

Gene Information

This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA which induces extensive excision repair mediated by uracil glycosylase. This repair process resulting in the removal and reincorporation of dUTP is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. [provided by RefSeq Jul 2008]

Gene Symbol:   DUT
Gene Name:   deoxyuridine triphosphatase
Aliases:   FLJ20622, dUTPase
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000128951
Entrez:   1854
Chromosome Mapping:   15q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.740000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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